Reporter

Part:BBa_K3112050:Design

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-15)


mRFP reporter with weak constitutive promoter(J23115)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal NheI site found at 16
    Illegal NheI site found at 39
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
    Illegal BamHI site found at 924
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EcoRI and BamHI sites at both ends of the part



Source

igem

References